By Helmut Breuninger, Patrick Adam

There hasn’t been a ebook touching on "Microscopically managed surgical procedure" released and it's important to submit a publication that info all of the diversified phrases and method utilized in microscopically managed surgical procedure. The objective is to create a pragmatic, concise and easy rationalization of 3D-histology with workflows and certain illustrative fabric for dermatologists. it really is for this reason designed to be a goal-oriented handbook instead of an exhaustive reference paintings. it's going to give you the crucial info for all operating with sufferers present process this workforce of treatments.

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Extra resources for 3D Histology Evaluation of Dermatologic Surgery

Sample text

The 12 o’clock orientation differs in the examples shown (Fig. 10a, a¢). 10 Vertical excision and square procedure, two possibilities of orientation cutting margins and base in a clockwise order Excising the Tumor 25 safety margin. From this square-shaped tumor specimen, the margins can now be easily cut away in a clockwise order (Fig. 10b). In the following, the bottom of the remaining specimen is separated and the tumor is sent for diagnostics (Fig. 10c). Now, embedding, horizontal slicing, and staining can begin, and after staining, tumor-positive areas can be detected (Fig.

This means that the loss in time is compensated by the requirement of fewer sections, being of higher quality. In the following, both methods will be discussed in detail. As before, all important procedures and steps are illustrated to help the reader. Paraffin Sectioning In this section, the preparation and sectioning of the tissue using paraffin embedding will be described. For paraffin sectioning, a prior formalin fixation of the samples is required. Usually the 30 Chapter 3. Embedding and Sectioning excised tissue is fixed immediately after excision and 3D histology can be performed on the prefixed material.

5 cm2 in a very good quality taking the whole plane of the wax block. This means that the loss in time is compensated by the requirement of fewer sections, being of higher quality. In the following, both methods will be discussed in detail. As before, all important procedures and steps are illustrated to help the reader. Paraffin Sectioning In this section, the preparation and sectioning of the tissue using paraffin embedding will be described. For paraffin sectioning, a prior formalin fixation of the samples is required.

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