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3D Histology Evaluation of Dermatologic Surgery by Helmut Breuninger, Patrick Adam

By Helmut Breuninger, Patrick Adam

There hasn’t been a e-book bearing on "Microscopically managed surgical procedure" released and it's important to post a publication that info the entire various phrases and technique utilized in microscopically managed surgical procedure. The aim is to create a pragmatic, concise and easy rationalization of 3D-histology with workflows and particular illustrative fabric for dermatologists. it really is as a result designed to be a goal-oriented handbook instead of an exhaustive reference paintings. it is going to give you the crucial details for all operating with sufferers present process this staff of treatments.

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3e). 1 Langenbrinck Corp. com 32 Chapter 3. 3 Embedding in a mold with paraffin to get a block for sectioning Preparation of Paraffin Sectioning The cooled and hardened paraffin block (Fig. 4a) has to be separated from the embedding mold by heating up shortly before the tissue can be sectioned in a microtome (Fig. 4b). The paraffin block with the embedded tissue will be strongly attached to the previously anchored embedding cassette and can therefore be used as a handle in the entire procedure.

3b). Then the tissue pieces are transferred into an embedding mold, pressing their outsides down (Fig. 3c, c¢). , the orientation of the samples must stay the same. Now the embedding mold can be filled with liquid paraffin (Fig. 3d), and the bottom of the embedding cassette is pressed into the still hot paraffin as a lit (Fig. 3e). 1 Langenbrinck Corp. com 32 Chapter 3. 3 Embedding in a mold with paraffin to get a block for sectioning Preparation of Paraffin Sectioning The cooled and hardened paraffin block (Fig.

7a). To separate the margin and central tumor from such specimens, the 2–3 mm wide margin strips can be removed using a scalpel and or scissors (Fig. 7a, b) so that the entire margin of 22 Chapter 2. 8 Two-dimensional schemes, comparing oblique (a) and vertical (b) incision and flattening of 3D margins the specimen is represented in one strip starting left from the 12 o’clock suture marker (Fig. 7b, c). This strip can then be laid flat with the outside of the surface facing down (Fig. 7c). Following the removal of the margins, the tumor center with its base was left behind (Fig.

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